Biochemistry Laboratory Techniques
本课程系统训练生物化学研究的基本实验技能和方法。实验内容包括蛋白质提取与纯化技术（盐析、透析、层析、电泳）、酶学实验（酶活力测定、动力学参数分析）、核酸实验（DNA/RNA提取、PCR、琼脂糖电泳）、分光光度法与色谱分析技术等。重点培养学生的实验操作能力、数据分析能力和科学思维，掌握常用生化仪器的使用方法，学会撰写规范的实验报告，为后续科研训练和毕业论文奠定实验基础。
This course provides systematic training in basic experimental skills and methods for biochemistry research. Experiments include protein extraction and purification techniques (salting out, dialysis, chromatography, electrophoresis), enzymology experiments (enzyme activity assay, kinetic parameter analysis), nucleic acid experiments (DNA/RNA extraction, PCR, agarose electrophoresis), spectrophotometry, and chromatographic analysis. Emphasis on developing experimental skills, data analysis ability, and scientific thinking.
学生需设计从植物材料中分离纯化过氧化物酶的完整实验方案，包括粗酶液制备、硫酸铵分级沉淀、透析脱盐、离子交换层析纯化等步骤。对纯化后的酶进行性质鉴定，包括SDS-PAGE纯度检测、分子量测定、最适pH和最适温度测定、米氏常数Km测定。报告需包含详细实验流程、原始数据记录、结果分析讨论及实验总结。
Students design a complete experimental protocol for isolating and purifying peroxidase from plant materials, including crude enzyme preparation, ammonium sulfate fractional precipitation, dialysis desalting, and ion exchange chromatography purification. The purified enzyme is characterized by SDS-PAGE purity detection, molecular weight determination, optimal pH and temperature measurement, and Michaelis constant Km determination. The report includes detailed experimental procedures, raw data records, result analysis, and experimental summary.