Structural Biochemistry
本课程从分子水平研究生物大分子的三维结构及其与功能的关系。内容涵盖蛋白质的氨基酸组成、一级结构测定、二级结构类型（α-螺旋、β-折叠）、三级结构与结构域、四级结构与亚基组装，以及核酸的双螺旋结构、超螺旋与染色体结构。重点讲授蛋白质折叠机制、分子伴侣、构象变化与功能调节，介绍X射线晶体学、核磁共振、冷冻电镜等结构生物学研究方法。
This course studies the three-dimensional structures of biological macromolecules and their relationship to function at the molecular level. Topics include protein amino acid composition, primary structure determination, secondary structure types (α-helix, β-sheet), tertiary structure and domains, quaternary structure and subunit assembly, as well as DNA double helix, supercoiling, and chromosome structure. Emphasis on protein folding mechanisms, chaperones, conformational changes, and functional regulation, with introduction to X-ray crystallography, NMR, and cryo-EM methods.
学生从PDB数据库中选择一个目标蛋白质结构文件，使用PyMOL或ChimeraX等分子可视化软件进行结构分析。内容包括展示蛋白质整体折叠、标注活性位点关键氨基酸残基、分析配体结合方式、比较同源蛋白结构差异。报告需包含结构图片、功能预测及结构与功能关系的讨论，掌握生物大分子结构分析的基本方法。
Students select a target protein structure from the PDB database and perform structural analysis using molecular visualization software such as PyMOL or ChimeraX. The project includes displaying overall protein folding, labeling key active site residues, analyzing ligand binding modes, and comparing homologous protein structures. The report includes structural images, function prediction, and discussion of structure-function relationships, mastering basic methods for biomolecular structure analysis.