Molecular Biology
本课程系统介绍分子生物学的基本原理与核心技术，包括DNA复制、转录、翻译及基因表达调控的分子机制。课程涵盖原核与真核生物的基因结构与基因组学基础，以及现代分子生物学研究方法如分子克隆、PCR、核酸杂交等技术原理。学生将掌握从分子水平理解生命现象的本质，为后续专业学习和科研实践奠定坚实的理论基础。
This course systematically introduces the fundamental principles and core techniques of molecular biology, including DNA replication, transcription, translation, and molecular mechanisms of gene expression regulation. It covers gene structure and genomics in prokaryotes and eukaryotes, as well as modern molecular biology research methods such as molecular cloning, PCR, and nucleic acid hybridization. Students will understand the nature of life at the molecular level.
选择一个感兴趣的人类基因，利用NCBI等数据库获取其序列信息，设计克隆方案，包括引物设计、酶切位点选择、载体构建策略，并分析该基因在不同组织中的表达模式。撰写完整的实验设计报告，包含技术路线图、预期结果分析及可能遇到的问题与解决方案。
Select a human gene of interest, retrieve its sequence information using databases such as NCBI, design a cloning strategy including primer design, restriction site selection, and vector construction strategy. Analyze the gene expression pattern in different tissues. Write a complete experimental design report.