Molecular Biology
本课程系统介绍分子生物学的基本原理与核心技术，包括DNA复制、转录、翻译及基因表达调控的分子机制。课程涵盖原核与真核生物的基因结构与基因组学基础，以及现代分子生物学研究方法如分子克隆、PCR、核酸杂交等技术原理。学生将掌握从分子水平理解生命现象的本质，为后续专业学习和科研实践奠定坚实的理论基础。
This course systematically introduces the fundamental principles and core techniques of molecular biology, including DNA replication, transcription, translation, and molecular mechanisms of gene expression regulation. It covers gene structure and genomics in prokaryotes and eukaryotes, as well as modern molecular biology research methods such as molecular cloning, PCR, and nucleic acid hybridization. Students will understand the nature of life at the molecular level.
选择一个目的基因，设计引物进行PCR扩增，构建原核表达载体，转化大肠杆菌并诱导表达，通过SDS-PAGE和Western Blot验证蛋白表达。
Select a target gene, design primers for PCR amplification, construct prokaryotic expression vector, transform E. coli and induce expression, verify protein expression by SDS-PAGE and Western Blot.