Molecular Genetics
本课程从分子水平探讨遗传信息的传递、表达与调控机制。内容包括基因突变与DNA修复、染色质结构与表观遗传、基因表达调控网络、基因组印记、非编码RNA功能等。课程还介绍现代遗传学研究技术如基因敲除、CRISPR基因编辑、转基因动物模型构建等。通过本课程学习，学生将深入理解遗传的分子基础及遗传病的发病机制。
This course explores the mechanisms of genetic information transmission, expression, and regulation at the molecular level. Topics include gene mutation and DNA repair, chromatin structure and epigenetics, gene expression regulatory networks, genomic imprinting, and non-coding RNA functions. The course also introduces modern genetic research techniques such as gene knockout and CRISPR gene editing.
选择一种与人类疾病相关的基因，设计利用CRISPR-Cas9技术对该基因进行敲除的完整实验方案。包括sgRNA设计原则与位点选择、载体构建、细胞转染与筛选策略、基因型鉴定方法以及功能验证实验设计。分析脱靶效应评估及实验对照设置，提交完整的实验设计报告。
Select a human disease-related gene and design a complete experimental protocol for gene knockout using CRISPR-Cas9 technology. Include sgRNA design principles and site selection, vector construction, cell transfection and screening strategies, genotyping methods, and functional validation experimental design.