Molecular Biology Techniques
本课程以实验操作为主，系统训练分子生物学常用实验技术。内容包括核酸提取与纯化、PCR扩增技术、琼脂糖凝胶电泳、限制性酶切与DNA连接、感受态细胞制备与转化、质粒提取、实时荧光定量PCR、蛋白质表达与纯化、Western Blot等。课程强调实验原理与操作技能并重，培养学生独立完成分子生物学实验的能力及分析解决实验问题的能力。
This course focuses on laboratory training, systematically teaching common molecular biology techniques. Topics include nucleic acid extraction and purification, PCR amplification, agarose gel electrophoresis, restriction digestion and DNA ligation, competent cell preparation and transformation, plasmid extraction, real-time qPCR, protein expression and purification, and Western Blot.
综合应用PCR、电泳、核酸杂交和基因测序技术，针对一种遗传病或病原体（如地中海贫血或新冠病毒）设计分子诊断方案，撰写完整检测报告。
Comprehensively apply PCR, electrophoresis, nucleic acid hybridization and gene sequencing techniques, design molecular diagnostic scheme for a genetic disease or pathogen (e.g., thalassemia or SARS-CoV-2), write complete detection report.